Identification of druggable hub genes and key pathways associated with cervical cancer by protein-protein interaction analysis: An in silico study
محل انتشار: مجله طب تولید مثل ایران، دوره: 21، شماره: 10
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 88
فایل این مقاله در 10 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJRM-21-10_004
تاریخ نمایه سازی: 19 آذر 1402
چکیده مقاله:
Background: The uncontrolled growth of abnormal cells in the cervix leads to cervical cancer (CC), the fourth most common gynecologic cancer. So far, many studies have been conducted on CC; however, it is still necessary to discover the hub gene, key pathways, and the exact underlying mechanisms involved in developing this disease.
Objective: This study aims to use gene expression patterns and protein-protein interaction (PPI) network analysis to identify key pathways and druggable hub genes in CC.
Materials and Methods: In this in silico analysis, ۲ microarray gene expression datasets; GSE۶۳۵۱۴ (۱۰۴ cancer and ۲۴ normal samples), and GSE۹۷۵۰ (۴۲ cancer and ۲۴ normal samples) were extracted from gene expression omnibus to identify common differentially expressed genes between them. Gene ontology and Kyoto encyclopedia of genes and genomes pathway analysis were performed via the Enrichr database. STRING ۱۲.۰ database and CytoHubba plugin in Cytoscape ۳.۹.۱ software were implemented to create and analyze the PPI network. Finally, druggable hub genes were screened.
Results: Based on the degree method, ۱۰ key genes were known as the hub genes after the screening of PPI networks by the CytoHubba plugin. NCAPG, KIF۱۱, TTK, PBK, MELK, ASPM, TPX۲, BUB۱, TOP۲A, and KIF۲C are the key genes, of which ۵ genes (KIF۱۱, TTK, PBK, MELK, and TOP۲A) were druggable.
Conclusion: This research provides a novel vision for designing therapeutic targets in patients with CC. However, these findings should be verified through additional experiments.
کلیدواژه ها:
نویسندگان
Azizeh Asadzadeh
Department of Biology, Faculty of Science, Nour Danesh Institute of Higher Education, Meymeh, Isfahan, Iran.
Nafiseh Ghorbani
Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
Katayoun Dastan
Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :